Shelf Life Determination of Instant Granules Combination of  Broccoli (Brassica oleracea L.) and Pegagan (Centella asiatica L.) Herb Extracts Using Arrhenius Model Accelerated Shelf Life Method

Wilda Nurhikmah; Erni Rustiani; Nabila Hidayat

Authors

Wilda Nurhikmah Department of Pharmacy, Universitas Pakuan, Bogor, Indonesia
Erni Rustiani Department of Pharmacy, Universitas Pakuan, Bogor, Indonesia
Nabila Hidayat Department of Pharmacy, Universitas Pakuan, Bogor, Indonesia

Keywords:

Accelerated Shelf Life Testing, broccoli, instant granule, pegagan, shelf life

Abstract

Dementia is one of the diseases whose number of sufferers has increased over the past 10 years. Some natural ingredients that can be used to improve memory are broccoli (Brassica oleracea L.) and pegagan (Centella asiatica L.) Urban). The high interest of Indonesian people in using herbs in the prevention of diseases is an opportunity to develop granule preparations of broccoli and pegagan extracts. Granules preparations are packaged in a sachet to make it easier to consume them. Physical quality testing and determination of its shelf life are carried out to ensure the quality of instant granules preparations. This study aims to evaluate the physical quality and determine the shelf life of instant granules using the Accelerated Shelf Life Testing (ASLT) method of the Arrhenius model. Preparation was carried out by wet granulation and then packaged using aluminum foil sachets. Furthermore, physical quality parameters and stability tests were evaluated with storage conditions at 40 °C, 45 °C, and 60 °C for 4 weeks. Parameters include: organoleptic, loss on drying (LOD), dispersion time, pH, and flavonoid content. The results of the evaluation of the physical quality of instant herbal granules combined with broccoli and pegagan extracts are LOD 4.4%, ash content 1.5%, flow rate 8.19 g/sec, angle of repose 25-30⁰, dispersion time 1 minute 24 seconds, pH 4.84, and flavonoid content 0.5 mg QE/g. The results of the stability test obtained data on the shelf life of instant granules for 13.2 weeks or 92 days at room temperature (25°C).

References

Alzobaidi, N., Quasimi, H., Emad, N. A., Alhalmi, A., & Naqvi, M. (2021). Bioactive Compounds and Traditional Herbal Medicine: Promising Approaches for the Treatment of Dementia. Degenerative neurological and neuromuscular disease, 11, 1–14. https://doi.org/10.2147/DNND.S299589.

Arif, A. B. (2016). Metode accelarated shelf life test (aslt) dengan pendekatan arrhenius dalam pendugaan umur simpan sari buah nanas, pepaya dan cempedak. Informatika Pertanian, 25(2), 189-198.

Asiah, N., Cempaka, L., & David, W. (2018). Panduan Praktis Pendugaan Umur Simpan Produk Pangan. Penerbitan Universitas Bakrie.

Beking, K., & Vieira, A. (2010). Flavonoid intake and disability-adjusted life years due to Alzheimer's and related dementias: a population-based study involving twenty-three developed countries. Public health nutrition, 13(9), 1403–1409. https://doi.org/10.1017/S1368980009992990.

BSN (1996), Serbuk Minuman Tradisional (SNI 01-4321-1996) Badan Standarisasi Nasional.

Cempaka, R. A., Santoso, S., & Tanuwijaya Laksmi Karunia. (2014). Pengaruh Metode Pengolahan (Juicing dan Blending) Terhadap Kandungan Quercetin Berbagai Varietas Apel Lokal dan Impor (Malus domestica). Indonesian Journal of Human Nutrition, 1(1), 14–22. www.ijhn.ub.ac.id

Chaudhary, R., & Kumari, P. (2022). Stability aspects of herbal formulation. World Journal of Pharmaceutical and Life Sciences, 8, 103-110.

Departemen Kesehatan Republik Indonesia. (1980). Materia Medika Indonesia Jilid IV. Jakarta: Direktorat Pengawasan Obat dan Makanan.

Kementerian Kesehatan Republik Indonesia. (2017). Farmakope Herbal Indonesia Edisi II. Jakarta: Kementerian Kesehatan Republik Indonesia..

Farooqui, A. A., Farooqui, T., Madan, A., Ong, J. H., & Ong, W. Y. (2018). Ayurvedic Medicine for the Treatment of Dementia: Mechanistic Aspects. Evidence-based complementary and alternative medicine : eCAM, 2018, 2481076. https://doi.org/10.1155/2018/2481076

Hohakay, J. J., Pontoh, J., & Yudistira, A. (2019). Pengaruh metode pengeringan terhadap kadar flavonoid daun sesewanua (clerodendron squamatum vahl.). Pharmacon, 8(3), 748-757.

Husni, P., Fadhiilah, M. L., & Hasanah, U. (2020). Formulasi Dan Uji Stabilitas Fisik Granul Instan Serbuk Kering Tangkai Genjer (Limnocharis flava (L.) Buchenau.) Sebagai Suplemen Penambah Serat. Jurnal Ilmiah Farmasi Farmasyifa, 3(1), 1-8.. J Ilm Farm Farmasyifa. 2020;3(1):1–8.

Laksmitawati, D., Nurhidayati, L., Arifin, M., & Bahtiar, B. (2017). Optimasi Konsentrasi Ekstrak dan Bahan Pengikat Polivinil Pirolidon pada Granul Ekstrak Daun Sirih Merah (Piper crocatum Ruiz & Pav) sebagai Antihiperurisemia. JURNAL ILMU KEFARMASIAN INDONESIA, 15(2), 216-222. https://doi:10.35814/jifi.v15i2.527

Luliana, S., Amalia, S., & Isnindar. (2023). Formulasi Serbuk Instan Ekstrak Pegagan (Centella asiatica) dan Jahe Merah (Zingiber Officinale Roscoe Var. Rubrum). Journal Syifa Sciences and Clinical Research (JSSCR), 5(3), 372–381. https://doi.org/10.37311/jsscr.v5i3.16499

Marlina, L., Kusumawati, A. H., Fikayuniar, L., Amalia, F., Aliani, N., & Rahmawati, I. (2021). Reformulasi Corigens dalam Sediaan Antiaging dan Joint Support Drink Mix Collagen Rousselot’s. Majalah Farmasetika, 6(1), 60–70

Monita, G., Mardiah, M., Jumiono, A., Akil, S., & Amalia, L. (2023). Formula dan Umur Simpan Minuman Serbuk Berbasis Rosela dengan Penyalut yang Berbeda. Jurnal Agroteknologi Vol, 17(02).

Nagraj, G.H, Chouksey, S, Jaiswal, S, Amit K. Jaiswal, (2020), Nutritional Composition and Antioxidant Properties of Fruits and Vegetables, Academic Press,

Nihaya,(2016) Kajian Potensi Efek Antidemensia Ekstrak Brokoli (Brassica oleracea L. var. Italica Plenck) dan Pegagan (Centella asiatica L. Urban) Pada Mencit Yang Diinduksi Skopolamin, Skripsi, Universitas Pakuan

Oktami, E., Lestari, F., & Aprilia, H. (2021). Studi Literatur Uji Stabilitas Sediaan Farmasi Bahan Alam. Prosiding Farmasi, 7(1), 73.

Putriana, N.A., , Mardawati,E., Wardhana,Y.W.,(2021), Formulation and Evaluation of instant granules from Ketapang Badak fruit (Ficus lyrata Warb) using wet granulation method as an antioxidant supplement, Indonesian Journal of Pharmaceutics , Vol 3, Issue 3, 2021 (116-127) https://doi.org/10.24198/idjp.v3i3.37062

Putri, Y. D., Warya, S., & Afdina, M. (2021). Formulasi Granul Instan Antioksidan Ekstrak Etanol Bawang Dayak (Eleutherine americana Merr.) dengan Variasi Pengikat PVP K30. Jurnal Sains Dan Teknologi Farmasi Indonesia, 10(2), 58–70. http://dx.doi.org/10.58327/jstfi.v10i2.157

Rani, K. C., Parfati, N., Muarofah, D., & Sacharia, S. N. (2020). Formulasi Granul Effervescent Herba Meniran (Phyllanthus niruri L.) dengan Variasi Suspending Agent Xanthan Gum, CMC-Na, dan Kombinasi CMC-Na-Mikrokristalin Selulosa RC-591. Jurnal Sains Farmasi & Klinis, 7(1), 39–51. https://doi.org/10.25077/jsfk.7.1.39-51.2020

Sabaragamuwa, Perera, Fedrizzi (2018), Centella asiatica (Pegagan) as a neuroprotectant and its potential role in healthy ageing, Trends in Food Science & Technology ,Volume 79, 2018, Pages 88-97, https://doi.org/10.1016/j.tifs.2018.07.024.

Sulistiawati, E. (2016). Formulasi Granul Instan Kombinasi Ekstrak Daging Buah Mahkota Dewa dan Daun Salam [Skripsi]. Universitas Pakuan

Syska, K., Ropiudin, Rudiyanri, E., Nurhayati, A.D., Wahdah, F., Estimation of Shelft life of Pegagan tea (Centella asiatica (L) Urban) Using Accelerated Shelf Life Testing (ASLT) Method with Arrhenius Model, Jurnal Keteknikan Pertanian dan Biosistem 11 (3)

World Health Organization, 1998, Quality control methods for medicinal plant materials, Geneva

Voigth R. (1994). Buku Pelajaran Teknologi Farmasi, Edisi Kelima. Penerjemah Drs. Soendani Noerono. Gadjah Mada University Perss. Yogyakarta.

Xu, P., Peng, X., Yuan, T., Yang, J., Li, X., Zhang, H., ... & Jia, X. (2021). Effect of vacuum drying on drying kinetics and quality of the aqueous extracts of Callicarpa nudiflora Hook. et Arn. Lwt, 152, 112305. https://doi.org/10.1016/j.lwt.2021.112305.