β-carotene Levels In Local Carrot  (Daucus Carota L.) Extract From Two Different Planting Areas

Euis Nining; Asri Wulandari; Trirakhma Sofihidayati; Imelda Fauzia Zulviany; Siti Aisyah

Authors

Euis Nining Department of Pharmacy, Universitas Pakuan, Bogor, Indonesia
Asri Wulandari Department of Pharmacy, Universitas Pakuan, Bogor, Indonesia
Trirakhma Sofihidayati Department of Pharmacy, Universitas Pakuan, Bogor, Indonesia
Imelda Fauzia Zulviany Department of Pharmacy, Universitas Pakuan, Bogor, Indonesia
Siti Aisyah Department of Pharmacy, Universitas Pakuan, Bogor, Indonesia

Keywords:

carrots, β-carotene, maceration, TLC, visible spectrophotometry

Abstract

Carrots or Daucus carota L. are vegetables that have many benefits. β-carotene as the main content in carrots can act as provitamin A, antioxidants, and skin and facial care. Two types of local carrots, namely carrots grown in Citeko, Bogor Regency and Sukatani, Cianjur Regency, have the potential to be developed as a source of natural β-carotene for drug and cosmetic preparations. Determination of β-carotene levels in local carrots needs to be done before being used as drug and cosmetic preparations. The purpose of this study was to determine the β-carotene levels in the extracts of two types of local carrots, namely carrots from Citeko, Bogor Regency and Sukatani, Cianjur Regency. The sample powder was extracted by the maceration method using n-hexane solvent (1:10). Qualitative β-carotene testing was carried out by the TLC method using a silica gel 60 F254 stationary phase and a petroleum ether: benzene mobile phase (9:1). Determination of β-carotene levels using a validated method, namely the visible light spectrophotometry method with n-hexane solvent at a wavelength of 450 nm. The results of this study indicate that the β-carotene levels in Citeko carrot extract are 86.94 ± 5.53 mg/g and 104.09 ± 4.21 mg/g for Sukatani carrot extract. Based on the results of the ANOVA test, the β-carotene levels in the two types of carrot extracts did not show significant differences. Both types of local carrot extracts, Citeko and Sukatani, have the potential as sources of β-carotene for drug and cosmetic preparations.

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