Effects of Dexamethasone on Locomotor Behavior in Adult Zebrafish Assessed Using AnimApp

Fibe Yulinda Cesa; Martanty Aditya; Mutiara  Fauziah; Noer Sidqi  Muhammadiy

doi:10.33751/jf.v16i1.67

Authors

Fibe Yulinda Cesa Department of Pharmacy, Universitas Ma Chung, Malang, Indonesia, 65151
Martanty Aditya Department of Pharmacy, Universitas Ma Chung, Malang, Indonesia, 65151
Mutiara Fauziah Department of Pharmacy, Universitas Ma Chung, Malang, Indonesia, 65151
Noer Sidqi Muhammadiy Department of Pharmacist Professional Study Program, Universitas Ma Chung, Malang, Indonesia

DOI:

https://doi.org/10.33751/jf.v16i1.67

Keywords:

animapp, corticosteroids, locomotor, side effect of depression, zebrafish

Abstract

Corticosteroids are drugs that contain steroids which are useful for supplementing steroid hormones in the body when needed, suppressing immune function, and reducing inflammation. Undoubtedly, corticosteroids are the most widely prescribed class of drugs worldwide. However, this medication is also known to have side effects in psychiatry, including depression. This study aims to determine the side effects of depression induced by the use of corticosteroid drugs, specifically dexamethasone, through locomotor behavior analysis using an adult zebrafish model (Danio rerio). The drug induction method in this study involved administering dexamethasone 20 µl intraperitoneally, targeting the peritoneum, the central metabolic organ of the Zebrafish. Using a sample of 30 Zebrafish divided into 3 test groups consisting of treatment and control groups. This study uses open-source multiplatform software (animapp) to measure and monitor changes in Zebrafish behavior over a 9-day observation period. (0; 3; 6; dan 9). The results of this study show a significant effect on freezing locomotor behavior after dexamethasone induction in the dexamethasone treatment group of Zebrafish compared to the negative control group (a-sig 0.007) and the dexamethasone treatment group compared to the positive control group (a-sig 0.047). A significant effect was also observed in movement locomotor behavior after dexamethasone induction in the dexamethasone treatment group compared to the positive control group (a-sig 0.002) and the dexamethasone treatment group compared to the negative control group (a-sig 0.045). The significant effects of both locomotor behaviors after dexamethasone induction reflect depressive symptoms characterized by decreased motor activity and increased immobility periods. Therefore, further monitoring of dexamethasone use is necessary, especially for patients suffering from depression, anxiety, and other psychiatric illnesses, to avoid exacerbating their existing depressive symptoms.

References

Ackerman, P. A., Morgan, J. D., & Iwama, G. K. (2005). Fish Anesthetics. CCAC Guidelines on: The Care and Use of Fish in Research, Teaching and Testing, 2005, Appendix, 1–22. https://ccac.ca/Documents/Standards/Guidelines/Add_PDFs/Fish_Anesthetics.pdf

Aditya, M. (2016). Kortikosteroid Induksi Sindrom Psikotik. Jurnal Wiyata, Vol. 3 No., 31–37.

Al Aziz, A. A. (2020). Hubungan Antara Intensitas Penggunaan Media Sosial dan Tingkat Depresi pada Mahasiswa. Acta Psychologia, 2(2), 92–107. https://doi.org/10.21831/ap.v2i2.35100

Appendix a : Anesthetic Agents for Use in Fish. (n.d.). d, 25.

Cooper, J. A., Arulpragasam, A. R., & Treadway, M. T. (2018). Anhedonia in depression: biological mechanisms and computational models. Current Opinion in Behavioral Sciences, 22, 128–135. https://doi.org/10.1016/j.cobeha.2018.01.024

Council, C. (2020). Canadian Council on Animal Care CCAC guidelines : Zebrafish and other small , warm-water laboratory fish.

Demin, K. A., Taranov, A. S., Ilyin, N. P., Lakstygal, A. M., Volgin, A. D., de Abreu, M. S., Strekalova, T., & Kalueff, A. V. (2021). Understanding neurobehavioral effects of acute and chronic stress in zebrafish. Stress (Amsterdam, Netherlands), 24(1), 1–18. https://doi.org/10.1080/10253890.2020.1724948

Deswati, D. A., Rohdiana, D., & Agustin, S. (2020). UJI EFEK DIURETIK SEDUHAN TEH PUTIH (Camellia seninsis L.) pada MENCIT PUTIH JANTAN GALUR SWISS WEBSTER. Jurnal Sabdariffarma, 9(1), 25–32. https://doi.org/10.53675/jsfar.v2i1.28

Egan, R. J., Bergner, C. L., Hart, P. C., Cachat, J. M., Canavello, P. R., Elegante, M. F., Elkhayat, S. I., Bartels, B. K., Tien, A. K., Tien, D. H., Mohnot, S., Beeson, E., Glasgow, E., Amri, H., Zukowska, Z., & Kalueff, A. V. (2009). Understanding behavioral and physiological phenotypes of stress and anxiety in zebrafish. Behavioural Brain Research, 205(1), 38–44. https://doi.org/10.1016/j.bbr.2009.06.022

Hindradjat, J., Ramli, M., & Radjah, C. L. (2021). Case Study On The Use of Cognitive Behavior Therapy (CBT) in Reducing Depression Levels in Adult Women. Jurnal Ilmu Keperawatan Jiwa, 4(2 SE-Articles), 263–272. https://journal.ppnijateng.org/index.php/jikj/article/view/992

Hodgens, A., & Sharman, T. (2024). Corticosteroids.

Kalueff, A., & Cachat, J. (2011). Zebrafish Neurobehavioral Protocols (Vol. 51). https://doi.org/10.1007/978-1-60761-953-6

Kumala, A. P., & Widianingtyas. (2018). EVALUASI PENGGUNAAN OBAT KORTIKOSTEROID DI APOTEK HS 23 PERIODE FEBRUARI – APRIL 2018. Jurnal Kefarmasian Akfarindo, 0(0 SE-Article). https://doi.org/10.37089/jofar.v0i0.38

Lesko, A., Kalafat, N., & Afreen, M. (2021). Four Case Reports of Acute Psychosis Secondary to Low Doses of Prednisone/Prednisolone. In Cureus (Vol. 13, Issue 12, p. e20853). https://doi.org/10.7759/cureus.20853

Morrow, S. A., Barr, J., Rosehart, H., & Ulch, S. (2015). Depression and hypomania symptoms are associated with high dose corticosteroids treatment for MS relapses. Journal of Affective Disorders, 187, 142–146. https://doi.org/10.1016/j.jad.2015.08.040

Muchdar, F., Juharni, J., & Andriani, R. (2020). Utilization of Different Probiotics on Growth and Survival Rate of Blacktail Zebra fish (Dascyllus melanurus). Agrikan: Jurnal Agribisnis Perikanan, 13(2), 222–231. https://doi.org/10.29239/j.agrikan.13.2.222-231

Putri, J. G., & Wisan, A. B. (2020). Efek Samping Terapi Kortikosteroid Sistemik Jangka Panjang pada Pasien Lupus Erimatosus Sistemik dan Tatalaksana Dermatologi. Cermin Dunia Kedokteran, 47(2 SE-Articles), 127–129. https://doi.org/10.55175/cdk.v47i2.282

Rao, S. R., Olechnowicz, S. W. Z., Krätschmer, P., Jepson, J. E. C., Edwards, C. M., & Edwards, J. R. (2019). Small Animal Video Tracking for Activity and Path Analysis Using a Novel Open-Source Multi-Platform Application (AnimApp). Scientific Reports, 9(1), 12343. https://doi.org/10.1038/s41598-019-48841-7

Skórzewska, A. (2021). Glucocorticoid-induced depression - the role of the dopaminergic system and microRNAs. Postepy Psychiatrii Neurologii, 30(3), 197–202. https://doi.org/10.5114/ppn.2021.110791

Thibaut, F. (2019). Corticosteroid-induced psychiatric disorders: genetic studies are needed. In European archives of psychiatry and clinical neuroscience (Vol. 269, Issue 6, pp. 623–625). https://doi.org/10.1007/s00406-019-01049-2