Antidiabetic Effectiveness Test of Combination of Soursop Leaf Extract and Red Betel Leaf Extract in Mice Induced By Alloxan

Eka Wahyu Riyadi; Gunawan Pamudji Widodo; Vivin Nopiyanti

doi:10.33751/jf.v16i1.72

Authors

Eka Wahyu Riyadi Universitas Setia Budi
Gunawan Pamudji Widodo Faculty of Pharmacy, Universitas Setia Budi, Surakarta, Central Java, Indonesia
Vivin Nopiyanti Faculty of Pharmacy, Universitas Setia Budi, Surakarta, Central Java, Indonesia

DOI:

https://doi.org/10.33751/jf.v16i1.72

Keywords:

Antidiabetic, soursop leaves, red betel leaves, alloxan, combination of extracts

Abstract

Soursop leaves contain saponins, terpenoids, steroids, flavonoids, tannins, and alkaloids, while red betel leaves contain flavonoids, polyphenols, saponins, alkaloids, and terpenoids (triterpenoids), which have the potential to lower blood glucose levels This study aims to determine the effect of the combination of soursop leaf extract and red betel leaf on changes in body weight and reduction of blood glucose levels in alloxan-induced mice, as well as to determine the most effective combination ratio. The study used 40 mice divided into eight groups: normal control, positive control (glibenclamide), negative control (Na-CMC), single soursop leaf extract, single red betel leaf extract, and three combination groups with ratios of 25%:75%, 50%:50%, and 75%:25%. Alloxan was injected intraperitoneally at a dose of 150 mg per kilogram of body weight, followed by treatment after 14 days. Blood glucose levels were measured on days 0, 3, 7, and 14 using a glucometer, and the data were analyzed using SPSS version 27. The results showed that both extracts contained alkaloids, flavonoids, tannins, saponins, and triterpenoids. The combination of extracts showed a tendency to improve body weight and lower blood glucose levels compared to the negative control. The 75%:25% ratio provided the highest blood glucose reduction descriptively, but statistical analysis showed no significant differences between the combination ratios. Thus, all combination ratios had relatively equivalent antidiabetic effectiveness.

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